8HQU
Crystal structure of the major sperm protein domain of SCS2 from saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.507, 68.507, 56.237 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.810 - 2.000 |
| R-factor | 0.2195 |
| Rwork | 0.218 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.783 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4778) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.966 |
| Rmeas | 0.079 | 0.992 |
| Rpim | 0.018 | 0.224 |
| Number of reflections | 10613 | 2607 |
| <I/σ(I)> | 28.32 | 3.38 |
| Completeness [%] | 99.9 | 99.81 |
| Redundancy | 19.3 | 19.5 |
| CC(1/2) | 1.000 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 25% PEG 3350, 0.1 mM tris-bis (pH 5.5) |
