8HPZ
Crystal structure of the MlaD domain of the MlaD protein from Escherichia coli (Form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-01-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 60.280, 107.290, 117.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.360 - 2.300 |
| R-factor | 0.22746 |
| Rwork | 0.225 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.867 |
| Data reduction software | MOSFLM (7.4.0) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.360 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | 0.595 |
| Rmeas | 0.088 | 0.636 |
| Rpim | 0.032 | 0.223 |
| Total number of observations | 137255 | 13294 |
| Number of reflections | 17387 | 1678 |
| <I/σ(I)> | 13.6 | 3.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.9 | 7.9 |
| CC(1/2) | 0.993 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 293 | 1.8 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic pH 5.0 |
