8HPX

Structure of mouse LGI1 LRR domain in space group P65

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 65
Unit cell lengths	90.952, 90.952, 191.228
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.480 - 3.680
R-factor	0.222
Rwork	0.220
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6K3S
RMSD bond length	0.003
RMSD bond angle	0.847
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.820
High resolution limit [Å]	3.680	3.680
Rmerge	0.059	0.959
Number of reflections	9697	445
<I/σ(I)>	39.866	2.415
Completeness [%]	98.9	100
Redundancy	6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.6M sodium citrate, 0.1M sodium cacodylate pH=6.5