8HPW
Crystal structure of mouse LGI1 LRR domain in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.158, 101.694, 65.848 |
| Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
| Resolution | 40.240 - 2.390 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w8a |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.841 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.077 | 0.480 |
| Number of reflections | 28150 | 1416 |
| <I/σ(I)> | 20.383 | 3.271 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 18% PEG 3000, 0.1M potassium/sodium phosphate pH=6.2, 0.2M sodium chloride |
