8HPP
Crystal structure of human INTS3 with SAGE1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 260.119, 46.241, 103.104 |
| Unit cell angles | 90.00, 113.28, 90.00 |
Refinement procedure
| Resolution | 47.400 - 3.000 |
| R-factor | 0.2173 |
| Rwork | 0.215 |
| R-free | 0.25730 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.089 | 0.031 | 0.518 |
| Total number of observations | 92299 | ||
| Number of reflections | 22718 | 2438 | 1940 |
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 97.4 | 99.5 | 84.1 |
| Redundancy | 4.1 | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 M Ammonium sulfate and 25% Ethylene Glycol 3350 |
