8HPK

Crystal structure of the bacterial oxalate transporter OxlT in an oxalate-bound occluded form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-17
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	114.950, 233.190, 50.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.100 - 3.000
R-factor	0.2406
Rwork	0.237
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pw4 1xf4
RMSD bond length	0.003
RMSD bond angle	0.584
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	2.940
High resolution limit [Å]	2.600	2.600
Number of reflections	22609	1130
<I/σ(I)>	16.4
Completeness [%]	92.9
Redundancy	99.8
CC(1/2)	0.997	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	0.1 M sodium citrate, pH 5.5, 0.05 M NaCl, 26% (v/v) PEG 400