8HPJ

Crystal structure of the bacterial oxalate transporter OxlT in a ligand-free outward-facing form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	76.870, 181.670, 81.170
Unit cell angles	90.00, 111.36, 90.00

Refinement procedure

Resolution	47.260 - 3.300
R-factor	0.261
Rwork	0.260
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hpk 5b3n
RMSD bond length	0.003
RMSD bond angle	0.594
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.260	3.400
High resolution limit [Å]	3.100	3.100
Number of reflections	26204	1310
<I/σ(I)>	5
Completeness [%]	88.4
Redundancy	24.8
CC(1/2)	0.980	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	9	293	100 mM Glycine, pH 9.0, 26-36% (v/v) PEG 400, 50-150 mM MnCl2