8HP3
Crystal structure of meso-diaminopimelate dehydrogenase from Prevotella timonensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-25 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.730, 189.600, 101.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.530 - 3.070 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.26051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.485 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 3.280 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmerge | 0.173 | 0.660 |
| Number of reflections | 18754 | 3351 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M HEPES, pH 7.5, 0.1 M ammonium sulfate |
