8HOV
Crystal structure of Hms1p from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.416, 99.175, 79.998 |
| Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
| Resolution | 33.660 - 2.770 |
| R-factor | 0.2355 |
| Rwork | 0.234 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4778) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.649 | |
| Rmeas | 0.913 | |
| Rpim | 0.371 | |
| Number of reflections | 28197 | 1224 |
| <I/σ(I)> | 16.47 | 1.74 |
| Completeness [%] | 94.6 | 91.6 |
| Redundancy | 3.3 | 2.7 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M Sodium citrate, pH 5.0, 8 % w/v PEG 8000 |
