8HNP
Archaeal transcription factor Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 123.170, 123.170, 82.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.840 - 3.390 |
| R-factor | 0.2395 |
| Rwork | 0.236 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.425 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.480 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Number of reflections | 14609 | 1065 |
| <I/σ(I)> | 16.86 | |
| Completeness [%] | 86.9 | 85.3 |
| Redundancy | 4.02 | |
| CC(1/2) | 0.998 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 30% PEG 400, 100 mM HEPES pH 7.5, and 200 mM ammonium sulfate |
