8HNJ
Domain-stabilized glutamine-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979490 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.100, 69.590, 83.740 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 28.790 - 2.030 |
| R-factor | 0.2278 |
| Rwork | 0.226 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.754 |
| Data reduction software | HKL-2000 |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.030 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 87318 | 9517 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.998 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG3350, 200 mM Potassium sodium tartrate |
