8HN9
Human SIRT3 Recognizing CCNE2K348la peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 235.676, 235.676, 235.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.230 - 3.700 |
| R-factor | 0.2331 |
| Rwork | 0.197 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.001 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 27432 | 893 |
| <I/σ(I)> | 14.47 | |
| Completeness [%] | 99.8 | |
| Redundancy | 20 | |
| CC(1/2) | 0.990 | 0.465 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.8 M succinic acid, pH 7.0, 0.01M Imidazole |
