8HN2
Selenomethionine-labelled soluble domain of Rieske iron-sulfur protein from chlorobaculum tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.640, 58.695, 49.680 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 46.050 - 2.300 |
| R-factor | 0.2074 |
| Rwork | 0.206 |
| R-free | 0.23150 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.322 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.18.2_3874)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.550 | 2.240 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.112 | 0.604 |
| Number of reflections | 15654 | 1581 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.2M ammonium sulfate, 0.1M ammonium citrate pH 5.0, 22% (w/v) Polyethylene Glycol 2000, 10%(v/v) ethanol |
