8HMG
The open state of RGLG2-VWA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 31 |
| Unit cell lengths | 244.410, 244.410, 63.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.810 - 2.810 |
| R-factor | 0.18468 |
| Rwork | 0.183 |
| R-free | 0.20816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.479 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.810 | 2.860 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Number of reflections | 103535 | 5094 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.43 | |
| CC(1/2) | 0.996 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 100 mM Tris-HCl, pH 7.5, 200 mM ammonium sulfate, 20% polyethylene glycol 4000 |
