8HJP

Crystal structure of glycosyltransferase SgUGT94-289-3 in complex with UDP state 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	63.742, 80.873, 173.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.840 - 2.200
R-factor	0.2301
Rwork	0.229
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jtd
RMSD bond length	0.006
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.840	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	46304	4553
<I/σ(I)>	15.91
Completeness [%]	99.7
Redundancy	12.8
CC(1/2)	0.999	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Tris-HCl, polyethylene glycol 3350, NaCl, Polypropylene glycol P400.