8HJG
Crystal structure of glycosyltransferase SgUGT94-289-3 in complex with M5, state 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.178, 76.483, 82.182 |
Unit cell angles | 90.00, 92.64, 90.00 |
Refinement procedure
Resolution | 19.650 - 1.700 |
R-factor | 0.1801 |
Rwork | 0.180 |
R-free | 0.19060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jtd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.650 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 102616 | 10112 |
<I/σ(I)> | 18.36 | |
Completeness [%] | 94.9 | |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | Tris-HCl, polyethylene glycol 3350, NaCl, Polypropylene glycol P400. |