8HHV
endo-alpha-D-arabinanase EndoMA1 from Microbacterium arabinogalactanolyticum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.285, 128.296, 104.019 |
| Unit cell angles | 90.00, 109.38, 90.00 |
Refinement procedure
| Resolution | 46.180 - 1.600 |
| R-factor | 0.17867 |
| Rwork | 0.177 |
| R-free | 0.21121 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.906 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | XDS (Jan 10, 2022) |
| Phasing software | PHENIX (1.12-2829) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.130 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | 0.485 |
| Rpim | 0.041 | 0.356 |
| Number of reflections | 261209 | 12725 |
| <I/σ(I)> | 9.9 | 1.9 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 3.8 | |
| CC(1/2) | 0.989 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 23% PEG 3350, 0.4 M sodium thiocyanate |
