8HGZ
Crystal structure of insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-17 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.5 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.885, 78.885, 79.272 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.640 - 1.700 |
| R-factor | 0.2214 |
| Rwork | 0.217 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xda |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.366 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.640 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | |
| Number of reflections | 110176 | 9012 |
| <I/σ(I)> | 23.33 | |
| Completeness [%] | 96.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M sodium acetate trihydrate, 0.1M tris hydrochloride pH 9 |
