8HGF
Human PKM2 mutant - C326S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 07A |
| Synchrotron site | NSRRC |
| Beamline | TPS 07A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.514, 156.373, 121.586 |
| Unit cell angles | 90.00, 114.20, 90.00 |
Refinement procedure
| Resolution | 29.790 - 3.100 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qg6 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.857 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.790 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.068 | 0.601 |
| Number of reflections | 55273 | 5394 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.4 M ammonium phosphate monobasic, 23% polyethylene glycerol 3350, Tris pH 7.0 |
