8HEH

Crystal structure of GCN5-related N-acetyltransferase 05790

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	C 1 2 1
Unit cell lengths	66.646, 68.373, 76.012
Unit cell angles	90.00, 98.12, 90.00

Refinement procedure

Resolution	19.500 - 1.400
Rwork	0.186
R-free	0.20590
Structure solution method	SAD
RMSD bond length	0.033
RMSD bond angle	1.218
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (v 1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	1.480
High resolution limit [Å]	1.400	1.400
Number of reflections	66387	9608
<I/σ(I)>	19.1
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	PEG 3000