8HCM

zebrafish IRF-11 DBD complex with DNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298.15
Detector technology	CCD
Collection date	2020-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 61 2 2
Unit cell lengths	61.118, 61.118, 220.054
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.270 - 2.590
R-factor	0.2356
Rwork	0.231
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2irf
RMSD bond length	0.011
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.270	2.690
High resolution limit [Å]	2.590	2.590
Number of reflections	12847	614
<I/σ(I)>	18.29
Completeness [%]	94.8
Redundancy	1.6
CC(1/2)	0.996	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	1.6 M sodium/potassium phosphate pH 6.5