8HAD
A novel dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 107.343, 115.468, 151.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.820 - 3.810 |
| R-factor | 0.3226 |
| Rwork | 0.321 |
| R-free | 0.36940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2woj |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.457 |
| Phasing software | CNS (v1.0) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.950 |
| High resolution limit [Å] | 3.810 | 3.810 |
| Rmerge | 0.076 | 1.016 |
| Number of reflections | 9530 | 931 |
| <I/σ(I)> | 24.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.07M sodium acetate, pH4.6, 5.6% PEG 4000, 30% Glycerol |
