8HAD
A novel dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 107.343, 115.468, 151.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.820 - 3.810 |
R-factor | 0.3226 |
Rwork | 0.321 |
R-free | 0.36940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2woj |
RMSD bond length | 0.002 |
RMSD bond angle | 0.457 |
Phasing software | CNS (v1.0) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.950 |
High resolution limit [Å] | 3.810 | 3.810 |
Rmerge | 0.076 | 1.016 |
Number of reflections | 9530 | 931 |
<I/σ(I)> | 24.1 | |
Completeness [%] | 99.9 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.07M sodium acetate, pH4.6, 5.6% PEG 4000, 30% Glycerol |