8HAC
A novel dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.297, 125.212, 120.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.700 - 2.320 |
| R-factor | 0.1955 |
| Rwork | 0.193 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2woj |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.785 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.037 | 0.644 |
| Number of reflections | 32898 | 3227 |
| <I/σ(I)> | 38.7 | |
| Completeness [%] | 98.0 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.085M sodium acetate, pH 4.7, 0.25M ammonium acetate, 18.5% glycerol, 0.25M sodium bromide, with 24.5% polyethylene glycol (PEG) 4000 |
