8H9Z

Annexin A5 protein mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	69.328, 134.651, 79.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.900 - 1.420
R-factor	0.231
Rwork	0.229
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1avh
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	1.470
High resolution limit [Å]	1.420	1.420
Rpim		0.500
Number of reflections	69662	2516
<I/σ(I)>	10.6
Completeness [%]	99.5
Redundancy	6.59
CC(1/2)	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 3350, sodium chloride, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid