8H91
Crystal structure of SARS-CoV-2 spike receptor-binding domain in complex with nanobody N19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-09-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.366, 57.366, 444.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.540 - 3.070 |
| R-factor | 0.2153 |
| Rwork | 0.212 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7w1s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.550 | 3.230 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmerge | 0.054 | 1.413 |
| Number of reflections | 14976 | 2124 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 98.9 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1%(w/v) Tryptone, 0.001 M Sodiumazide, 0.05 M HepesSodium pH7.0, 20%(w/v) polyethyleneglycol3350 |
