8H8V

Room-temperature structure of lysozyme by pink-beam serial crystallography (100 ms, edge)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	299.15
Detector technology	PIXEL
Collection date	2022-09-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8009
Spacegroup name	P 43 21 2
Unit cell lengths	78.880, 78.880, 38.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.780 - 1.700
R-factor	0.2275
Rwork	0.226
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wuc
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.600	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	14189	1380
<I/σ(I)>	6.73
Completeness [%]	100.0
Redundancy	1256.7
CC(1/2)	0.989	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		298.5	Na-acetate, NaCl, PEG 8000