8H8Q
Fab-amyloid beta fragment complex at neutral pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.040, 94.150, 115.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.257 - 2.500 |
Rwork | 0.212 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7e6p |
RMSD bond length | 0.001 |
RMSD bond angle | 0.511 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.961 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 15801 | 1530 |
<I/σ(I)> | 9.1 | |
Completeness [%] | 100.0 | |
Redundancy | 35.1 | |
CC(1/2) | 0.983 | 0.739 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | PEG3350, magnesium chloride, HEPES pH 7.2 |