8H7J
The crystal structure of CD163 SRCR5-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.328, 60.085, 85.424 |
| Unit cell angles | 90.00, 114.12, 90.00 |
Refinement procedure
| Resolution | 48.487 - 2.500 |
| Rwork | 0.182 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jfb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.437 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.139 | 1.552 |
| Rpim | 0.059 | 0.779 |
| Number of reflections | 20350 | 1962 |
| <I/σ(I)> | 21.1 | 2.2 |
| Completeness [%] | 98.7 | 96.6 |
| Redundancy | 6.4 | 4.4 |
| CC(1/2) | 0.996 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 4000, 0.15M potassium sodium tartrate tetra-hydrate pH 8.5 |
