8H70
Crystal structure of the catalytic ATP-binding domain of the PhoR sensor histidine kinase from Vibrio cholera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-02 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.258, 90.953, 87.521 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.480 - 1.880 |
R-factor | 0.2086 |
Rwork | 0.206 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.873 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PARROT |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.480 | 1.990 |
High resolution limit [Å] | 1.880 | 1.880 |
Number of reflections | 94388 | 4275 |
<I/σ(I)> | 7.51 | |
Completeness [%] | 99.6 | |
Redundancy | 3.5 | |
CC(1/2) | 0.997 | 0.492 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | NaCl, BICINE, PEG 300 |