8H6D
Crystal structure of human GCN5 histone acetyltransferase domain bound with glutaryl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2015-12-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 3 |
Unit cell lengths | 175.265, 175.265, 175.265 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.610 - 3.260 |
R-factor | 0.1792 |
Rwork | 0.176 |
R-free | 0.23550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5trm |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.250 | 8.810 | 3.250 |
Rmerge | 0.174 | 0.052 | 0.613 |
Rmeas | 0.187 | 0.056 | 0.659 |
Rpim | 0.067 | 0.020 | 0.238 |
Total number of observations | 213058 | ||
Number of reflections | 28347 | 1510 | 1394 |
<I/σ(I)> | 4 | ||
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 7.5 | 6.9 | 7.6 |
CC(1/2) | 0.997 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.3M Ammonium sulfate, 0.4M Lithium chloride |