8H6D

Crystal structure of human GCN5 histone acetyltransferase domain bound with glutaryl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	77
Detector technology	CCD
Collection date	2015-12-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 3
Unit cell lengths	175.265, 175.265, 175.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.610 - 3.260
R-factor	0.1792
Rwork	0.176
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5trm
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.250	8.810	3.250
Rmerge	0.174	0.052	0.613
Rmeas	0.187	0.056	0.659
Rpim	0.067	0.020	0.238
Total number of observations	213058
Number of reflections	28347	1510	1394
<I/σ(I)>	4
Completeness [%]	100.0	99.4	100
Redundancy	7.5	6.9	7.6
CC(1/2)		0.997	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.3M Ammonium sulfate, 0.4M Lithium chloride