8H66

Crystal structure of human GCN5 histone acetyltransferase domain bound with propionyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	77
Detector technology	CCD
Collection date	2015-10-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 3
Unit cell lengths	176.182, 176.182, 176.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.860 - 2.800
R-factor	0.1889
Rwork	0.186
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5trm
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.128	0.051	0.507
Rmeas	0.132	0.052	0.520
Rpim	0.029	0.012	0.114
Total number of observations	914919
Number of reflections	44869	2377	2222
<I/σ(I)>	6.9
Completeness [%]	100.0	99.9	100
Redundancy	20.4	18.2	20.9
CC(1/2)		1.000	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.4M Ammonium sulfate, 0.1M Sodium acetate pH 4.6