8H56

Crystal structure of Rep' of porcine circovirus type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-03
Detector	Nonius Kappa CCD
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	73.120, 73.120, 133.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.000 - 2.330
R-factor	0.254
Rwork	0.253
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xor
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.240	2.390
High resolution limit [Å]	2.330	2.330
Rmerge		0.444
Number of reflections	18281	1287
<I/σ(I)>	22.9
Completeness [%]	99.7
Redundancy	14.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M HEPES, pH 7.0, 0.1M Sodium chloride, 1.5 M Ammonium sulfate