8H4A

Blasnase-T13A/M57P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	173
Detector technology	CCD
Collection date	2021-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	91.672, 91.672, 233.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.840 - 1.900
Rwork	0.172
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c8x
RMSD bond length	0.009
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.270	1.940
High resolution limit [Å]	1.900	1.900
Rpim	0.047	0.242
Number of reflections	79441	4448
<I/σ(I)>	10.8
Completeness [%]	100.0
Redundancy	25.3
CC(1/2)	0.996	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	sodium formate