8H47
Blasnase-T13A/P55F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2021-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.513, 92.513, 230.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 1.900 |
| Rwork | 0.192 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c8x |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.980 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.023 | 0.652 |
| Number of reflections | 72525 | 4842 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 90.8 | |
| Redundancy | 25.1 | |
| CC(1/2) | 1.000 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | sodium formate |
