8H3T
The crystal structure of AlpH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-04-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.209, 94.812, 110.108 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.406 - 1.866 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MoRDA |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.850 | 1.970 |
High resolution limit [Å] | 1.866 | 1.870 |
Rmerge | 0.110 | 0.957 |
Number of reflections | 59235 | 5551 |
<I/σ(I)> | 17.6 | |
Completeness [%] | 98.6 | |
Redundancy | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 289 | 0.03 M citric acid, 0.07 M BIS-TRIS propane (pH 7.6), 20% (w/v) PEG3350 |