8H3B

Crystal structure of antibody scFv against M2e Influenza peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 42 21 2
Unit cell lengths	106.973, 106.973, 149.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.640 - 2.750
R-factor	0.1779
Rwork	0.181
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsi
RMSD bond length	0.003
RMSD bond angle	0.662
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (dev_4753)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.640	2.800
High resolution limit [Å]	2.750	2.750
Number of reflections	23027	1110
<I/σ(I)>	12.9	3.4
Completeness [%]	99.6
Redundancy	10
CC(1/2)	0.995	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	4M Formate