8H2F
Crystal structure of DnaQ domain in complex witn TMP of Streptococcus thermophilus strain DGCC 7710
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97855 |
Spacegroup name | H 3 |
Unit cell lengths | 96.139, 96.139, 61.419 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.713 - 1.448 |
R-factor | 0.1759 |
Rwork | 0.175 |
R-free | 0.18810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IJ53 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.480 |
High resolution limit [Å] | 1.448 | 3.940 | 1.450 |
Rmerge | 0.105 | 0.072 | 0.801 |
Rmeas | 0.110 | 0.076 | 0.856 |
Rpim | 0.034 | 0.023 | 0.295 |
Number of reflections | 37677 | 1883 | 1879 |
<I/σ(I)> | 5.4 | ||
Completeness [%] | 100.0 | 99.9 | 99.8 |
Redundancy | 10.1 | 10.9 | 7.9 |
CC(1/2) | 0.995 | 0.763 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200mM ammonium sulfate, 36% (w/v) PEG8000, 100mM sodium cacodylate trihydrate pH 6.5 |