8H20

Serine Palmitoyltransferase from Sphingobacterium multivorum complexed with Glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	61.573, 61.573, 207.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.059 - 1.450
Rwork	0.188
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a2b
RMSD bond length	0.013
RMSD bond angle	1.894
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.540
High resolution limit [Å]	1.450	1.450
Number of reflections	72011	11434
<I/σ(I)>	22.8
Completeness [%]	100.0
Redundancy	9.6
CC(1/2)	0.999	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4000, sodium acetate, Tris-HCl