8H1Y
Serine Palmitoyltransferase from Sphingobacterium multivorum complexed with L-homoserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-12-23 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.238, 61.238, 208.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.950 - 1.550 |
| Rwork | 0.183 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a2b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.698 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 57736 | 8613 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 98.3 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.997 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | PEG4000, sodium acetate, Tris-HCl |
