8H1Q

Serine Palmitoyltransferase from Sphingobacterium multivorum complexed with L-serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	61.282, 61.282, 207.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.940 - 1.500
Rwork	0.148
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a2b
RMSD bond length	0.012
RMSD bond angle	1.829
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	64186	10153
<I/σ(I)>	15
Completeness [%]	99.8
Redundancy	9.3
CC(1/2)	0.998	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4000, sodium acetate, Tris-HCl