8H0H

Hypotethical protein from Mycobacterium tuberculsosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	55.197, 87.518, 55.907
Unit cell angles	90.00, 94.51, 90.00

Refinement procedure

Resolution	47.010 - 2.740
R-factor	0.2024
Rwork	0.200
R-free	0.25410
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.740	2.750
Number of reflections	13538	636
<I/σ(I)>	8.1
Completeness [%]	96.4
Redundancy	4.5
CC(1/2)	0.960	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis/Tris Propane/HCl, pH 6.5, 0.2 M sodium fluoride and 20% (w/v) polyethylene glycol 3350