8GZ0
Structure of hypothetical protein TTHA1873 with phosphate from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-07-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 90.010, 90.010, 140.671 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.270 - 2.220 |
| R-factor | 0.1761 |
| Rwork | 0.174 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wwo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.238 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.270 | 2.290 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 17209 | 1271 |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 98.2 | |
| Redundancy | 33.5 | |
| CC(1/2) | 0.998 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 60% v/v Tacismate |
