8GYZ

Crystal structure of transcription factor TGA7 from Arabidopsis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	73.755, 85.840, 89.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.942 - 2.060
R-factor	0.1843
Rwork	0.182
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q93ZE2-F1
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.940	2.170
High resolution limit [Å]	2.060	2.060
Number of reflections	59814	4419
<I/σ(I)>	8.1
Completeness [%]	91.9
Redundancy	10
CC(1/2)	0.996	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.005M magnesium sulfate heptahydrate, 0.05 M MES monohydrate pH 6.0, 5% w/v polyethylene glycol 4000, 0.2 M magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400.