8GYY

Bifunctional xylosidase/glucosidase LXYL with intermediate substrate xylose, 120 seconds

Replaces:  7F95

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	228.429, 89.230, 218.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.970 - 2.070
R-factor	0.22109
Rwork	0.219
R-free	0.25895
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kj0
RMSD bond length	0.008
RMSD bond angle	1.532
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	158.240	2.110
High resolution limit [Å]	2.068	2.068
Rmerge	0.112	0.913
Rmeas	0.132
Rpim	0.067	0.555
Number of reflections	257788	12570
<I/σ(I)>	30.8
Completeness [%]	95.1
Redundancy	3.5	3.3
CC(1/2)		0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.16	13% PEG3350, 0.1 M Tris-HCl pH8.5, 0.2% MgCl2