8GYR
Crystal structure of a variable region segment of Leptospira host-interacting outer surface protein, LigA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-03-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.219, 63.905, 171.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.920 - 1.870 |
| R-factor | 0.20885 |
| Rwork | 0.208 |
| R-free | 0.23411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mog |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.337 |
| Data reduction software | DIALS |
| Data scaling software | pointless |
| Phasing software | BUCCANEER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.920 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.113 | |
| Number of reflections | 31943 | 1705 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.8 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.994 | 0.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 23% PEG 3350, 260mM Potassium Iodide and 100mM Bis-Tris Propane |
