8GYJ

Crystal structure of Fic25 complexed with PLP-(5S,6S)-N2-acetyl-DADH adduct from Streptomyces ficellus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	201.443, 201.443, 57.003
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.430 - 1.820
R-factor	0.1604
Rwork	0.159
R-free	0.18510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gyh
RMSD bond length	0.003
RMSD bond angle	1.229
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	1.850
High resolution limit [Å]	1.820	1.820
Rmerge	0.113	0.980
Number of reflections	118807	5871
<I/σ(I)>	17.8
Completeness [%]	100.0
Redundancy	10.4
CC(1/2)	0.999	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.1 M Sodium citrate, 0.1 M Imidazole, pH 8.0