8GYF
Crystal structure of a bright green fluorescent protein (StayGold) with single mutation (K192Y) in jellyfish Cytaeis uchidae from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.946418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 134.707, 134.707, 58.568 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.235 - 2.000 |
| Rwork | 0.192 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF2 predicted |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.529 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.235 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.261 | 1.097 |
| Number of reflections | 40718 | 2994 |
| <I/σ(I)> | 7.9 | 2.4 |
| Completeness [%] | 98.8 | |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium formate, 20% PEG 3350 |
