8GYC

Annexin A5 protein dimer mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	69.198, 79.743, 133.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.980 - 1.800
R-factor	0.2592
Rwork	0.258
R-free	0.28850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1avh
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.145	0.092	1.953
Rmeas	0.151	0.096	2.043
Rpim	0.043	0.028	0.590
Total number of observations	854364
Number of reflections	68560	3682	3399
<I/σ(I)>	2.7
Completeness [%]	99.9	99.4	99.8
Redundancy	12.5	12	11.5
CC(1/2)		0.996	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 3350, sodium chloride, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid