8GVB

The complex between public TCR TD08 and HLA-A24 bound to HIV-1 Nef138-8 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 32 2 1
Unit cell lengths	71.657, 71.657, 381.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.010 - 3.200
R-factor	0.2821
Rwork	0.280
R-free	0.32080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vxm
RMSD bond length	0.005
RMSD bond angle	0.630
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.318
High resolution limit [Å]	3.200	3.200
Rmerge	0.147	2.172
Number of reflections	19722	19722
<I/σ(I)>	19.031	1.48
Completeness [%]	99.4	100
Redundancy	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.06 M divalent ions (MgCl2, CaCl2), 0.1 M Tris (pH 8.5), 0.1 M Bicine (pH 8.5)