8GV7

Crystal structure of PN-SIA28 in complex with influenza hemagglutinin H18 A/flat-faced bat/Peru/033/2010

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 63
Unit cell lengths	140.427, 140.427, 195.734
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.970 - 2.600
R-factor	0.2095
Rwork	0.209
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjs
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.160	1.680
Number of reflections	66862	6653
<I/σ(I)>	19.83	1.66
Completeness [%]	94.1
Redundancy	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Bis Tris 25 % w/v PEG 3350(pH 5.5)